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Professor David Klenerman

Abstract:

We develop novel biophysical methods based on single molecule fluorescence and super-resolution imaging and use a scanned nanopipette to deliver controlled numbers of molecules to cells to initiate signalling . We focus on using these methods to image individual biological molecules on or in live cells as they perform their functions and offer PhD projects which allow students to exploit them to study a wide range of different biological problems to gain new insights and observe processes, undetectable by conventional bulk methods.     We are currently studying  the organisation of signalling proteins on the cell surface that give rise to the innate and immune response  and how they reorganise to give rise to signalling on live cells, how the proteins misfold to form cytotoxic  aggregates and how these aggregates interact with and  damage neuronal cells,  following how individual ubiquinated proteins are degraded by the proteasome, observing  DNA replication in live S.Pombe at the single molecule level  and  imaging  the organisation of chromatin in embryonic stem cells at 20 nm resolution

References:

Ubiquitin chain conformation regulates recognition and activity of interacting proteins. Nature  492, 266-270 (2012).    Direct Observation of the Interconversion of Normal and Toxic Forms of alpha-Synuclein. Cell  149, 1048-1059 (2012).    Imaging the cell surface and its organization down to the level of single molecules.   Philosophical transactions of the Royal Society of London. Series B, Biological sciences (2013) 368, 20120027

Professor David Klenerman

Professor David Klenerman
Department of Chemistry
Office Phone: 01223 336481