The project will study the structure and mechanism of drug efflux pumps from bacteria that are driven by ATP hydrolysis. The pumps are three-component assemblies, and protocols have been established in our laboratory to prepare stable specimens that are suitable for structure analysis by cryo-electron microscopy and X-ray crystallography and functional analysis in reconstituted transport assays.
- Bavro, V.N., Pietras, Z., Furnham, N., Perez-Cano, L., Fernandez-Recio, J., Pei, X.Y., Misra, R. and Luisi, B.F. (2008) Channel-opening and assembly in a bacterial drug efflux machine. Molecular Cell 30, 114-121.
- Bandyra, K.J., Said, N., Pfeiffer, V., Gorna, M.W., Vogel, J., Luisi, B.F. (2012) The seed region of a small RNA drives the controlled destruction of the target mRNA by the endoribonuclease RNase E. Mol. Cell 47, 943-953.
- Hardwick, S.W., Gubbey, T., Hug, I., Jenal, U., Luisi, B.F. (2012) Crystal structure of Caulobacter crescentus polynucleotide phosphorylase reveals a mechanism of RNA substrate channelling and RNA degradosome assembly. Open Biol 2, 120028.