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Congratulations to Alzbeta Cardova on her publication in the Journal of Pharmaceutical and Biomedical Analysis

last modified Nov 10, 2017 03:07 PM

Congratulations to Alzbeta Cardova, 2014 cohort PhD student in the BBSRC DTP Programme, for publishing the paper Electrochemical and optical study of metallothionein interactions with prion proteins in the Journal of Pharmaceutical and Biomedical Analysis.

Abstract

The prion protein (PrPC) can be structurally shifted to its PrPSc isoform causing a wide range of neurodegenerative diseases, which are currently incurable. There is an evidence that metallothioneins (MTs), and especially MT-3, are associated with neurodegenerative diseases. PrPC and MTs play pivotal roles in maintaining metal homeostasis; therefore, it is conceivable that each of them has its own significance in prion diseases. In this paper, we study the nature of interactions between PrPC, MT, and copper ions, Cu(II), using the method of differential pulse voltammetry (DPV) coupled with adsorptive transfer stripping technique (AdTS). Electrochemical properties of PrP itself and its interactions with both the Cu(II) ions and MTs have been found. Based on the results obtained, we hypothesised the formation of the complex in molar ratio 2:1 (PrPC:MT). Surface plasmon resonance imaging (SPRi) was used as a control reference assay to further confirm results obtained by the electrochemical approach, such as the specific interactions between PrPC and MT-3.

Read the full paper.

 

Alzbeta is researching the identification of genetic modifiers of prion replication and neurotoxicity in the lab of Dr Raymond Bujdoso in the Department of Veterinary Medicine at the University of Cambridge. As part of the BBSRC DTP Programme she completed rotation projects in Veterinary Medicine and at the Institute of Metabolic Science with Dr Giles Yeo. Alzbeta is currently undertaking an internship at Acidophil.