Congratulations to Celia Alda-Catalinas, 2015 cohort PhD student in the BBSRC DTP Programme, for publishing the paper ‘scNMT-seq enables joint profiling of chromatin accessibility DNA methylation and transcription in single cells’ in Nature Communications.
AbstractParallel single-cell sequencing protocols represent powerful methods for investigating regulatory relationships, including epigenome-transcriptome interactions. Here, we report a single-cell method for parallel chromatin accessibility, DNA methylation and transcriptome profiling. scNMT-seq (single-cell nucleosome, methylation and transcription sequencing) uses a GpC methyltransferase to label open chromatin followed by bisulfite and RNA sequencing. We validate scNMT-seq by applying it to differentiating mouse embryonic stem cells, finding links between all three molecular layers and revealing dynamic coupling between epigenomic layers during differentiation. |
